Category Archives: Research papers

Papers from our group that get published.

Probing Gαi1 protein activation at single-amino acid resolution

We present comprehensive maps at single-amino acid resolution of the residues stabilizing the human Gαi1 subunit in nucleotide- and receptor-bound states. We generated these maps by measuring the effects of alanine mutations on the stability of Gαi1 and the rhodopsin-Gαi1 … Continue reading

Posted in Research papers | Comments Off

Variable Glutamine-Rich Repeats Modulate Transcription Factor Activity

Excessive expansions of glutamine (Q)-rich repeats in various human proteins are known to result in severe neurodegenerative disorders such as Huntington’s disease and several ataxias. However, the physiological role of these repeats and the consequences of more moderate repeat variation remain unknown. Here, we demonstrate that … Continue reading

Posted in Research papers | Comments Off

Universal allosteric mechanism for Ga activation by GPCRs

G protein-coupled receptors (GPCRs) allosterically activate heterotrimeric G proteins and trigger GDP release. Given that there are 800 human GPCRs and 16 different Ga genes, this raises the question of whether a universal allosteric mechanism governs Ga activation. Here we show that … Continue reading

Posted in Research papers | Comments Off

Sequence composition of disordered regions fine-tunes protein half-life

The proteasome controls the concentrations of most proteins in eukaryotic cells. It recognizes its protein substrates through ubiquitin tags and initiates degradation at disordered regions within the substrate. Here we show that the proteasome has pronounced preferences for the amino … Continue reading

Posted in Research papers | Comments Off

Proteome response at the edge of protein aggregation

Proteins adopt defined structures and are crucial to most cellular functions. Their misfolding and aggregation is associated with numerous degenerative human disorders such as type II diabetes, Huntington’s or Alzheimer’s diseases. Here, we aim to understand why cells promote the … Continue reading

Posted in Research papers | Comments Off

Optimizing membrane-protein biogenesis through nonoptimal-codon usage

Two studies provide insights into the distinct strategies used by prokaryotes and eukaryotes to pause translation in order to facilitate cotranslational targeting of membrane proteins to the translocon. The review by Alexey Morgunov can be viewed here.

Posted in Research papers | Comments Off

Structured and disordered facets of the GPCR fold

The seven-transmembrane (7TM) helix fold of G-protein coupled receptors (GPCRs) has been adapted for a wide variety of physiologically important signaling functions. Here, we discuss the diversity in the structured and disordered regions of GPCRs based on the recently published … Continue reading

Posted in Research papers | Comments Off

Intrinsically Disordered Segments Affect Protein Half-Life in the Cell and during Evolution

Precise control of protein turnover is essential for cellular homeostasis. The ubiquitin-proteasome system is well established as a major regulator of protein degradation, but an understanding of how inherent structural features influence the lifetimes of proteins is lacking. We report … Continue reading

Posted in Research papers | Comments Off

Asymmetric mRNA localization contributes to fidelity and sensitivity of spatially localized systems

Although many proteins are localized after translation, asymmetric protein distribution is also achieved by translation after mRNA localization. Why are certain mRNA transported to a distal location and translated on-site? Here we undertake a systematic, genome-scale study of asymmetrically distributed … Continue reading

Posted in Research papers | Comments Off

A Million Peptide Motifs for the Molecular Biologist

A molecular description of functional modules in the cell is the focus of many high-throughput studies in the postgenomic era. A large portion of biomolecular interactions in virtually all cellular processes is mediated by compact interaction modules, referred to as … Continue reading

Posted in Research papers | Comments Off